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SRX10121671: RNA-seq of Phalaenopsis equestris: Labellum Biological replicate 2
1 ILLUMINA (Illumina HiSeq 4000) run: 35.4M spots, 2.2G bases, 738.9Mb downloads

Design: RNA was extracted using the RNeasy mini kit (Qiagen, The Netherlands) following the manufacturers protocol. To ensure a high quality of Phalaenopsis samples, RNA was analyzed using capillary electrophoresis on Agilent Bioanalyzer 2100. cDNA libraries for Illumina sequencing were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs, MA, USA) following the manufacturers protocol in 0.5 of the recommended volume (due to low RNA quantity in such samples as shoot apical meristem). cDNA libraries were sequenced with the HiSeq4000 and NextSeq500 (Illumina, CA, USA) instruments (50 bp and 75 bp single read run).
Submitted by: The Institute for Information Transmission Problems
Study: Transcriptome atlas of Phalaenopsis equestris
show Abstracthide Abstract
The vast diversity of Orchidaceae together with sophisticated adaptations to pollinators and other unique features make this family an attractive model for evolutionary and functional studies. The sequenced genome of Phalaenopsis equestris facilitates Orchidaceae research. Here we present an RNA-seq based transcriptome map of P. equestris which covers 19 organs of the plant including leaves, roots, floral organs and shoot apical meristem. We demonstrated the high quality of the data and showed the similarity of P. equestris transcriptome map with gene expression atlases of other plants. The transcriptome map can be easily accessed through our database Transcriptome Variation Analysis (TraVA) visualizing gene expression profiles. As an example of the application we analyzed the expression of Phalaenopsis "orphan" genes - the ones that do not have recognizable similarity with genes of other plants. We found that about a half of them are not expressed; the ones that are expressed have a predominant expression pattern in reproductive structures.
Sample: Phalaenopsis equestris Labellum
SAMN17840129 • SRS8276346 • All experiments • All runs
Library:
Name: Pheq_F.LAB.y_rep1
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: Oligo-dT
Layout: SINGLE
Runs: 1 run, 35.4M spots, 2.2G bases, 738.9Mb
Run# of Spots# of BasesSizePublished
SRR1373411135,359,3052.2G738.9Mb2021-09-13

ID:
13249247

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